Short peptides derived from the interaction domain of SARS coronavirus nonstructural protein nsp10 can suppress the 2'-O-methyltransferase activity of nsp10/nsp16 complex.
Identifieur interne : 001C52 ( Main/Exploration ); précédent : 001C51; suivant : 001C53Short peptides derived from the interaction domain of SARS coronavirus nonstructural protein nsp10 can suppress the 2'-O-methyltransferase activity of nsp10/nsp16 complex.
Auteurs : Min Ke [République populaire de Chine] ; Yu Chen ; Andong Wu ; Ying Sun ; Ceyang Su ; Hao Wu ; Xu Jin ; Jiali Tao ; Yi Wang ; Xiao Ma ; Ji-An Pan ; Deyin GuoSource :
- Virus research [ 1872-7492 ] ; 2012.
Descripteurs français
- KwdFr :
- Antienzymes (métabolisme), Cartographie d'interactions entre protéines, Conformation des protéines, Methyltransferases (antagonistes et inhibiteurs), Methyltransferases (métabolisme), Modèles moléculaires, Motifs et domaines d'intéraction protéique, Peptides (métabolisme), Protéines virales non structurales (antagonistes et inhibiteurs), Protéines virales non structurales (métabolisme), Techniques de double hybride, Virus du SRAS (enzymologie).
- MESH :
- antagonistes et inhibiteurs : Methyltransferases, Protéines virales non structurales.
- enzymologie : Virus du SRAS.
- métabolisme : Antienzymes, Methyltransferases, Peptides, Protéines virales non structurales.
- Cartographie d'interactions entre protéines, Conformation des protéines, Modèles moléculaires, Motifs et domaines d'intéraction protéique, Techniques de double hybride.
English descriptors
- KwdEn :
- Enzyme Inhibitors (metabolism), Methyltransferases (antagonists & inhibitors), Methyltransferases (metabolism), Models, Molecular, Peptides (metabolism), Protein Conformation, Protein Interaction Domains and Motifs, Protein Interaction Mapping, SARS Virus (enzymology), Two-Hybrid System Techniques, Viral Nonstructural Proteins (antagonists & inhibitors), Viral Nonstructural Proteins (metabolism).
- MESH :
- chemical , antagonists & inhibitors : Methyltransferases, Viral Nonstructural Proteins.
- chemical , metabolism : Enzyme Inhibitors, Methyltransferases, Peptides, Viral Nonstructural Proteins.
- enzymology : SARS Virus.
- Models, Molecular, Protein Conformation, Protein Interaction Domains and Motifs, Protein Interaction Mapping, Two-Hybrid System Techniques.
Abstract
Coronaviruses are the etiological agents of respiratory and enteric diseases in humans and livestock, exemplified by the life-threatening severe acute respiratory syndrome (SARS) caused by SARS coronavirus (SARS-CoV). However, effective means for combating coronaviruses are still lacking. The interaction between nonstructural protein (nsp) 10 and nsp16 has been demonstrated and the crystal structure of SARS-CoV nsp16/10 complex has been revealed. As nsp10 acts as an essential trigger to activate the 2'-O-methyltransferase activity of nsp16, short peptides derived from nsp10 may have inhibitory effect on viral 2'-O-methyltransferase activity. In this study, we revealed that the domain of aa 65-107 of nsp10 was sufficient for its interaction with nsp16 and the region of aa 42-120 in nsp10, which is larger than the interaction domain, was needed for stimulating the nsp16 2'-O-methyltransferase activity. We further showed that two short peptides derived from the interaction domain of nsp10 could inhibit the 2'-O-methyltransferase activity of SARS-CoV nsp16/10 complex, thus providing a novel strategy and proof-of-principle study for developing peptide inhibitors against SARS-CoV.
DOI: 10.1016/j.virusres.2012.05.017
PubMed: 22659295
Affiliations:
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Le document en format XML
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Laboratory of Virology and Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan 430072</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName></affiliation></author><author><name sortKey="Chen, Yu" sort="Chen, Yu" uniqKey="Chen Y" first="Yu" last="Chen">Yu Chen</name></author><author><name sortKey="Wu, Andong" sort="Wu, Andong" uniqKey="Wu A" first="Andong" last="Wu">Andong Wu</name></author><author><name sortKey="Sun, Ying" sort="Sun, Ying" uniqKey="Sun Y" first="Ying" last="Sun">Ying Sun</name></author><author><name sortKey="Su, Ceyang" sort="Su, Ceyang" uniqKey="Su C" first="Ceyang" last="Su">Ceyang Su</name></author><author><name sortKey="Wu, Hao" sort="Wu, Hao" uniqKey="Wu H" first="Hao" last="Wu">Hao Wu</name></author><author><name sortKey="Jin, Xu" sort="Jin, Xu" uniqKey="Jin X" first="Xu" last="Jin">Xu Jin</name></author><author><name sortKey="Tao, Jiali" sort="Tao, Jiali" uniqKey="Tao J" first="Jiali" last="Tao">Jiali Tao</name></author><author><name sortKey="Wang, Yi" sort="Wang, Yi" uniqKey="Wang Y" first="Yi" last="Wang">Yi Wang</name></author><author><name sortKey="Ma, Xiao" sort="Ma, Xiao" uniqKey="Ma X" first="Xiao" last="Ma">Xiao Ma</name></author><author><name sortKey="Pan, Ji An" sort="Pan, Ji An" uniqKey="Pan J" first="Ji-An" last="Pan">Ji-An Pan</name></author><author><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name></author></analytic><series><title level="j">Virus research</title><idno type="eISSN">1872-7492</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Enzyme Inhibitors (metabolism)</term><term>Methyltransferases (antagonists & inhibitors)</term><term>Methyltransferases (metabolism)</term><term>Models, Molecular</term><term>Peptides (metabolism)</term><term>Protein Conformation</term><term>Protein Interaction Domains and Motifs</term><term>Protein Interaction Mapping</term><term>SARS Virus (enzymology)</term><term>Two-Hybrid System Techniques</term><term>Viral Nonstructural Proteins (antagonists & inhibitors)</term><term>Viral Nonstructural Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antienzymes (métabolisme)</term><term>Cartographie d'interactions entre protéines</term><term>Conformation des protéines</term><term>Methyltransferases (antagonistes et inhibiteurs)</term><term>Methyltransferases (métabolisme)</term><term>Modèles moléculaires</term><term>Motifs et domaines d'intéraction protéique</term><term>Peptides (métabolisme)</term><term>Protéines virales non structurales (antagonistes et inhibiteurs)</term><term>Protéines virales non structurales (métabolisme)</term><term>Techniques de double hybride</term><term>Virus du SRAS (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Methyltransferases</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Enzyme Inhibitors</term><term>Methyltransferases</term><term>Peptides</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Methyltransferases</term><term>Protéines virales non structurales</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antienzymes</term><term>Methyltransferases</term><term>Peptides</term><term>Protéines virales non structurales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Models, Molecular</term><term>Protein Conformation</term><term>Protein Interaction Domains and Motifs</term><term>Protein Interaction Mapping</term><term>Two-Hybrid System Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie d'interactions entre protéines</term><term>Conformation des protéines</term><term>Modèles moléculaires</term><term>Motifs et domaines d'intéraction protéique</term><term>Techniques de double hybride</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Coronaviruses are the etiological agents of respiratory and enteric diseases in humans and livestock, exemplified by the life-threatening severe acute respiratory syndrome (SARS) caused by SARS coronavirus (SARS-CoV). However, effective means for combating coronaviruses are still lacking. The interaction between nonstructural protein (nsp) 10 and nsp16 has been demonstrated and the crystal structure of SARS-CoV nsp16/10 complex has been revealed. As nsp10 acts as an essential trigger to activate the 2'-O-methyltransferase activity of nsp16, short peptides derived from nsp10 may have inhibitory effect on viral 2'-O-methyltransferase activity. In this study, we revealed that the domain of aa 65-107 of nsp10 was sufficient for its interaction with nsp16 and the region of aa 42-120 in nsp10, which is larger than the interaction domain, was needed for stimulating the nsp16 2'-O-methyltransferase activity. We further showed that two short peptides derived from the interaction domain of nsp10 could inhibit the 2'-O-methyltransferase activity of SARS-CoV nsp16/10 complex, thus providing a novel strategy and proof-of-principle study for developing peptide inhibitors against SARS-CoV.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><noCountry><name sortKey="Chen, Yu" sort="Chen, Yu" uniqKey="Chen Y" first="Yu" last="Chen">Yu Chen</name><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name><name sortKey="Jin, Xu" sort="Jin, Xu" uniqKey="Jin X" first="Xu" last="Jin">Xu Jin</name><name sortKey="Ma, Xiao" sort="Ma, Xiao" uniqKey="Ma X" first="Xiao" last="Ma">Xiao Ma</name><name sortKey="Pan, Ji An" sort="Pan, Ji An" uniqKey="Pan J" first="Ji-An" last="Pan">Ji-An Pan</name><name sortKey="Su, Ceyang" sort="Su, Ceyang" uniqKey="Su C" first="Ceyang" last="Su">Ceyang Su</name><name sortKey="Sun, Ying" sort="Sun, Ying" uniqKey="Sun Y" first="Ying" last="Sun">Ying Sun</name><name sortKey="Tao, Jiali" sort="Tao, Jiali" uniqKey="Tao J" first="Jiali" last="Tao">Jiali Tao</name><name sortKey="Wang, Yi" sort="Wang, Yi" uniqKey="Wang Y" first="Yi" last="Wang">Yi Wang</name><name sortKey="Wu, Andong" sort="Wu, Andong" uniqKey="Wu A" first="Andong" last="Wu">Andong Wu</name><name sortKey="Wu, Hao" sort="Wu, Hao" uniqKey="Wu H" first="Hao" last="Wu">Hao Wu</name></noCountry><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Ke, Min" sort="Ke, Min" uniqKey="Ke M" first="Min" last="Ke">Min Ke</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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